Form 8-K

8-K — Aprea Therapeutics, Inc.

Accession: 0001104659-26-094542

Filed: 2026-08-12

Period: 2026-08-12

CIK: 0001781983

SIC: 2834 (PHARMACEUTICAL PREPARATIONS)

Item: Results of Operations and Financial Condition

Item: Other Events

Item: Financial Statements and Exhibits

Documents

8-K — apre-20260812x8k.htm (Primary)

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8-K

8-K (Primary)

Filename: apre-20260812x8k.htm · Sequence: 1

Aprea Therapeutics, Inc._August 12, 2026

0001781983false00017819832026-08-122026-08-12

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM 8-K

CURRENT REPORT

Pursuant to Section 13 or 15(d)

of the Securities Exchange Act of 1934

August 12, 2026

Date of Report (Date of earliest event reported)

Aprea Therapeutics, Inc.

(Exact name of registrant as specified in its charter)

Delaware

001-39069

84-2246769

(State or other jurisdiction

of incorporation)

(Commission

File Number)

(IRS Employer

Identification No.)

3805 Old Easton Road

Doylestown, PA

(Address of principal executive offices)

18902

(Zip Code)

Registrant’s telephone number, including area code: (215) 948-4119

(Former name or former address, if changed since last report): Not applicable

Check the appropriate box below if the Form 8-K filing is intended to simultaneously satisfy the filing obligation of the registrant under any of the following provisions:

☐

Written communications pursuant to Rule 425 under the Securities Act (17 CFR 230.425)

☐

Soliciting material pursuant to Rule 14a-12 under the Exchange Act (17 CFR 240.14a-12)

☐

Pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act (17 CFR 240.14d-2(b))

☐

Pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act (17 CFR 240.13e-4(c))

Securities registered pursuant to Section 12(b) of the Act:

Title of each class

Trading Symbol(s)

Name of each exchange on

which registered

Common stock, par value $0.001 per share

APRE

The Nasdaq Stock Market LLC

Indicate by check mark whether the registrant is an emerging growth company as defined in Rule 405 of the Securities Act of 1933 (§230.405 of this chapter) or Rule 12b-2 of the Securities Exchange Act of 1934 (§240.12b-2 of this chapter).

Emerging growth company ☐

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐

Item 2.02Results of Operations and Financial Condition.

On August 12, 2026, Aprea Therapeutics, Inc. (the “Company”) issued a press release announcing its financial results for the three and six months ended June 30, 2026, and provided an update on the Company’s operations for the same period. The Company is furnishing a copy of the press release, which is attached hereto as Exhibit 99.1.

In accordance with General Instruction B.2 of Form 8-K, the information included in this Item 2.02, including Exhibit 99.1 hereto, shall not be deemed “filed” for the purposes of Section 18 of the Securities Exchange Act of 1934, as amended (the “Exchange Act”), or otherwise subject to the liabilities of that section, nor shall it be deemed incorporated by reference into any filing made by the Company under the Exchange Act or Securities Act of 1933, as amended, except as shall be expressly set forth by specific reference in such a filing.

Item 8.01Other Events.

On August 12, 2026, the Company updated its corporate presentation slide deck. A copy of the corporate presentation slide deck is filed as Exhibit 99.2 hereto and incorporated herein by reference.

Item 9.01Financial Statements and Exhibits.

(d) Exhibits.

Exhibit

Number

Description

99.1

Press release issued by Aprea Therapeutics, Inc. dated August 12, 2026.

99.2

Corporate Presentation (August 2026).

104

Cover Page Interactive Data File (embedded within the inline XBRL document).

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned hereunto duly authorized.

Aprea Therapeutics, Inc.

Dated: August 12, 2026

By:

/s/ Oren Gilad

Name:

Oren Gilad, Ph.D.

Title:

President and Chief Executive Officer

EX-99.1

Filename: apre-20260812xex99d1.htm · Sequence: 2

Exhibit 99.1

Aprea Therapeutics Announces Second Quarter 2026 Financial Results

Enrollment in the ongoing Phase 1 dose escalation ACESOT-1051 trial of oral WEE1 inhibitor APR-1051 is accelerating as dose escalation advances

Aprea plans to advance APR-1051 in uterine serous carcinoma (USC), and Cyclin E–overexpressing platinum-resistant ovarian cancer (PROC) while also evaluating combination regimens with standard of care in HPV-positive head and neck cancer and colorectal cancer

Updated Phase 1 data presented at ASCO 2026 showed early single-agent activity for APR-1051, including partial responses and disease stabilization, with a manageable tolerability profile

$41.2 million in cash and cash equivalents as of June 30, 2026

DOYLESTOWN, PA, August 12, 2026 (GLOBE NEWSWIRE) — Aprea Therapeutics, Inc. (Nasdaq: APRE) (“Aprea”, or the “Company”), a clinical-stage precision medicine oncology company focused on the discovery and development of targeted therapies for patients with biomarker-defined cancers,

today reported financial results for the second quarter ended June 30, 2026, and provided a business update.

"Having observed early signs of clinical activity for APR-1051, we are now expanding the ongoing ACESOT-1051 trial,” said Oren Gilad, Ph.D., President and Chief Executive Officer of Aprea. “We are accelerating enrollment, advancing dose escalation to inform dose selection, and preparing to evaluate APR-1051 both as a single agent in uterine serous carcinoma (USC) and Cyclin E-overexpressing platinum-resistant ovarian cancer (PROC) and also in combination with standard of care in HPV-positive head and neck cancer and colorectal cancer. We look forward to sharing our next clinical data update at a medical meeting in the fourth quarter of 2026. This ongoing progress reflects our commitment to developing targeted cancer therapies that have the potential to improve outcomes and quality of life for patients, while also creating value for our shareholders."

Key Business Updates and Upcoming Key Milestones

ACESOT-1051: A Biomarker-Focused, Phase 1 Trial of Oral WEE1 Inhibitor, APR-1051

• APR-1051 is a potent and selective, oral small molecule WEE1 inhibitor designed to potentially address therapeutic window limitations observed with earlier WEE1 programs. It is currently being evaluated in ACESOT-1051, a multi-center, open-label Phase 1 study. The primary objectives of this study are safety, dose-limiting toxicity, maximum tolerated or maximum administered dose, and

RP2D. Secondary objectives include pharmacokinetics and antitumor activity assessed by RECIST/PCWG3.

• Aprea expects to report the next clinical data update from ACESOT-1051 at a medical meeting in the fourth quarter of 2026. Enrollment is accelerating ahead of this anticipated clinical catalyst, with the number of active clinical sites expanding from three to ten. The Company expects enrollment to reach 6 to 10 patients per month by Q4 of 2026, potentially increasing the pace of clinical data generation.

• Supported by the $30 million private placement that closed in the first quarter of 2026, the Company is expanding enrollment in ACESOT to include at least 50 patients with uterine serous carcinoma or cyclin E-overexpressing, platinum-resistant ovarian cancer. Completion of dose escalation and backfill expansion is anticipated in the second quarter of 2027. This expansion is intended to further characterize the clinical activity of APR-1051 in biomarker-defined tumor populations with a mechanistic rationale for WEE1 inhibition.

• Aprea also plans to evaluate APR-1051 in combination settings, supported by preclinical synergy observed in relevant disease models. In HPV-positive head and neck squamous cell carcinoma, APR-1051 will be combined with immune checkpoint therapy. In colorectal cancer, APR-1051 will be combined with a standard-of-care chemotherapy. Advancing clinically evaluated, active doses are intended to support the tolerability and dosing of APR-1051 when added to standard-of-care treatment.

• For more information on ACESOT-1051, refer to ClinicalTrials.gov NCT06260514.

APR-1051 Presentation at ASCO 2026

• On May 30, 2026, Aprea presented updated data from ACESOT-1051 in a poster titled “Early results from the first-in-human phase 1 study of WEE1 inhibitor APR-1051 in patients with advanced solid tumors (ACESOT-1051)” (Abstract #3107) at the American Society of Clinical Oncology (ASCO) 2026 Annual Meeting in Chicago, Illinois. The presentation summarized data as of a May 6, 2026 cutoff. A copy of the poster can be found on the Aprea corporate website here.

ATR inhibitor, ATRN-119

• ATRN-119 is a potent and highly selective potentially first-in-class macrocyclic ATR inhibitor designed for patients with tumors harboring mutations in DDR-related genes. Cancers with mutations in DDR-related genes represent a high unmet medical need, and these patients often have a poor prognosis and currently lack effective therapeutic options.

• During 2025, Aprea determined the recommended Phase 2 monotherapy dose (RP2D) of 1,100 mg once daily for ATRN-119 in the ABOYA-119 Phase 1/2a dose-escalation study and subsequently closed this study to focus resources on the clinical development of APR-1051. Building on the completion of dose escalation, the Company is considering further ATRN-119 development in combination approaches that could expand its therapeutic potential. Aprea believes ATRN-119's mechanism of action, potentially favorable safety profile, and pharmacologic characteristics could make it an ideal candidate for combination with other anti-cancer therapies, including radiation therapy, chemotherapy, antibody-drug conjugates (ADCs) and immune checkpoint inhibitors.

• Aprea is currently in discussions with leading academic centers to explore various combinations for ATRN-119. These include investigator-initiated studies evaluating ATRN-119 in combination with I/O

agents, chemotherapy, ADCs and/or radiation. Potential indications for these combinations include advanced solid tumors (e.g. HPV+ head and neck cancers, sarcomas, ovarian, colorectal, lung) and hematologic malignancies (e.g. Acute Myeloid Leukemia, Myelodysplastic syndromes).

Pipeline

• Aprea also has an early-stage program, a macrocyclic DYRK1A/B inhibitor, that potentially could enter IND enabling studies in the fourth quarter of 2026, subject to available resources.

Financial Results for the Second Quarter Ended June 30, 2026

• As of June 30, 2026, the Company reported cash and cash equivalents of $41.2 million, compared to $14.6 million as of December 31, 2025. The Company believes that its cash and cash equivalents as of June 30, 2026 will be sufficient to meet its currently projected operating expenses and capital expenditure requirements into the first quarter of 2028.

• For the second quarter ended June 30, 2026, the Company reported an operating loss of $4.0 million, compared to an operating loss of $3.4 million in the second quarter of 2025.

• Research and Development (R&D) expenses were $2.5 million for the quarter ended June 30, 2026, compared to $1.9 million for the second quarter of 2025. The increase in R&D expense was primarily related to higher expenses in ACESOT-1051, our Phase 1 dose-escalation study for APR-1051, partially offset by lower expense in the ABOYA-119 clinical trial to evaluate ATRN-119, which has been closed.

• General and Administrative (G&A) expenses were $1.6 million for each of the quarters ended June 30, 2026 and 2025.

• The Company reported a net loss of $3.6 million, or $(0.07) per basic share, on approximately 53.5 million weighted-average common shares outstanding for the quarter ended June 30, 2026, compared to a net loss of $3.2 million, or $(0.53) per basic share, on approximately 6.1 million weighted-average common shares outstanding for the comparable period in 2025.

About Aprea

Aprea is a clinical-stage precision medicine oncology company focused on the discovery and development of targeted therapies for patients with biomarker-defined cancers. The Company is pioneering a new approach to treat cancer by exploiting vulnerabilities associated with cancer cell mutations. This approach was developed to kill tumors while minimizing the effect on normal, healthy cells. Aprea’s technology has potential applications across multiple cancer types, enabling it to target a range of tumors, including ovarian, endometrial, colorectal and head and neck squamous cell carcinoma. The company’s lead programs are APR-1051, an oral, small-molecule inhibitor of WEE1 kinase, and ATRN-119, a small molecule ATR inhibitor, both in clinical development for solid tumor indications. For more information, please visit the company website at www.aprea.com.

The Company may use, and intends to use, its investor relations website at https://ir.aprea.com/ as a means of disclosing material nonpublic information and for complying with its disclosure obligations under Regulation FD.

Forward-Looking Statement

Certain information contained in this press release includes “forward-looking statements”, within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, related to our study analyses, clinical trials, regulatory submissions, development plans and projected cash position. We may, in some cases, use terms such as “future,” “predicts,” “believes,” “potential,” “continue,” “anticipates,” “estimates,” “expects,” “plans,” “intends,” “targeting,” “confidence,” “may,” “could,” “might,” “likely,” “will,” “should” or other words that convey uncertainty of future events or outcomes to identify these forward-looking statements. Our forward-looking statements are based on current beliefs and expectations of our management team and on information currently available to management, and involve risks, potential changes in circumstances, assumptions and uncertainties. All statements contained in this press release other than statements of historical fact are forward-looking statements, including statements regarding our ability to develop, commercialize and achieve market acceptance of our current and planned product candidates, our research and development efforts, including timing considerations and other matters regarding our business strategies, use of capital, results of operations and financial position, projected cash runway, plans and objectives for future operations, the timing, progress, enrollment and completion of our ongoing and planned clinical trials, the timing of expected clinical data updates and medical meeting presentations, the potential expansion of ACESOT-1051, the potential development of APR-1051 in single-agent and combination settings, the potential further development of ATRN-119, and the potential advancement of our early-stage programs. Any or all of the forward-looking statements may turn out to be wrong or be affected by inaccurate assumptions we might make or by known or unknown risks and uncertainties. These forward-looking statements are subject to risks and uncertainties including, without limitation, risks related to the success, timing and cost of our ongoing and anticipated clinical trials for our current product candidates, including the timing of initiation, pace of enrollment and completion of trials, our ability to fully fund our disclosed clinical trials, which assumes no material changes to our currently projected expenses, the receipt and timing of interim or preliminary results, which are not necessarily indicative of final results, our understanding of product candidate mechanisms of action and interpretation of preclinical and early clinical results from our clinical development programs, our ability to predict clinical outcomes based on such preclinical and early clinical results, the timing and outcome of regulatory interactions, and the other risks, uncertainties and factors described under “Risk Factors,” “Management’s Discussion and Analysis of Financial Condition and Results of Operations” and elsewhere in the documents we file with the U.S. Securities and Exchange Commission. For all these reasons, actual results and developments could be materially different from those expressed in or implied by our forward-looking statements. You are cautioned not to place undue reliance on these forward-looking statements, which are made only as of the date of this press release. We undertake no obligation to update such forward-looking statements for any reason, except as required by law.

Investor Contact:

Mike Moyer

LifeSci Advisors

mmoyer@lifesciadvisors.com

Aprea Therapeutics, Inc.

Consolidated Balance Sheets

June 30, 2026

December 31, 2025

Assets

(unaudited)

Current assets:

Cash and cash equivalents

41,233,428

14,599,347

Prepaid expenses and other current assets

679,686

961,899

Total current assets

41,913,114

15,561,246

Property and equipment, net

64,033

59,807

Restricted cash

41,615

41,186

Other noncurrent assets

271,162

Total assets

42,289,924

15,933,401

Liabilities and Stockholders’ Equity

Current liabilities:

Accounts payable

935,139

713,668

Accrued expenses

2,157,163

2,050,690

Total current liabilities

3,092,302

2,764,358

Commitments and contingencies

Series A convertible preferred stock, $0.001 par value, 40,000,000 shares authorized; 31,194 shares issued and outstanding at June 30, 2026 and December 31, 2025

727,361

Stockholders’ equity:

Common stock, $0.001 par value, 400,000,000 shares authorized, 12,391,136 and 8,192,538 shares issued and outstanding at June 30, 2026 and December 31, 2025, respectively

12,391

8,192

Additional paid-in capital

389,596,149

356,709,645

Accumulated other comprehensive loss

(10,617,617)

(10,634,714)

Accumulated deficit

(340,520,662)

(333,641,441)

Total stockholders’ equity

38,470,261

12,441,682

Total liabilities and stockholders' equity

42,289,924

15,933,401

Aprea Therapeutics, Inc.

Consolidated Statements of Operations and Comprehensive Loss

(Unaudited)

Three Months Ended June 30,

Six Months Ended June 30,

2026

2025

2026

2025

Grant revenue

—

118,111

—

280,574

Operating expenses:

Research and development

2,462,042

1,912,213

4,073,209

4,395,279

General and administrative

1,570,138

1,593,671

3,389,383

3,358,650

Total operating expenses

4,032,180

3,505,884

7,462,592

7,753,929

Loss from operations

(4,032,180)

(3,387,773)

(7,462,592)

(7,473,355)

Other income (expense):

Interest income, net

358,923

178,027

493,707

382,753

Other income

79,000

—

79,000

—

Foreign currency gain (loss)

2,953

(29,124)

10,664

(80,927)

Total other income

440,876

148,903

583,371

301,826

Net loss

(3,591,304)

(3,238,870)

(6,879,221)

(7,171,529)

Other comprehensive loss:

Foreign currency translation

8,083

(1,681)

17,097

(1,038)

Total comprehensive loss

(3,583,221)

(3,240,551)

(6,862,124)

(7,172,567)

Net loss per share attributable to common stockholders, basic and diluted

(0.07)

(0.53)

(0.20)

(1.19)

Weighted-average common shares outstanding, basic and diluted

53,483,504

6,083,329

34,191,653

6,038,845

EX-99.2

Filename: apre-20260812xex99d2.htm · Sequence: 3

Exhibit 99.2

A clinical-stage precision

medicine oncology company

focused on the discovery and

development of targeted

therapies for patients with

biomarker-defined cancers

August 2026

Forward-Looking Statements

Certain information contained in this presentation includes “forward-looking statements”, within the meaning of Section 27A of the Securities Act of 1933, as

amended, and Section 21E of the Securities Exchange Act of 1934, as amended related to our study analyses, clinical trials, regulatory submissions, and projected

cash position. We may, in some cases use terms such as “future,” “predicts,” “believes,” “potential,” “continue,” “anticipates,” “estimates,” “expects,” “plans,”

“intends,” “targeting,” “confidence,” “may,” “could,” “might,” “likely,” “will,” “should” or other words that convey uncertainty of the future events or outcomes to identify

these forward-looking statements. Our forward-looking statements are based on current beliefs and expectations of our management team and on information

currently available to management that involve risks, potential changes in circumstances, assumptions, and uncertainties. All statements contained in this

presentation other than statements of historical fact are forward-looking statements, including statements regarding our ability to develop, commercialize, and

achieve market acceptance of our current and planned product candidates, our research and development efforts, including timing considerations and other

matters regarding our business strategies, use of capital, results of operations and financial position, project cash runway, plans and objectives for future

operations, the timing, progress, enrollment and completion of our ongoing and planned clinical trials, the timing of expected clinical data updates and medical

meeting presentations, the potential expansion of ACESOT-1051, the potential development of APR-1051 in single-agent and combination settings, the potential

further development of ATRN-119, and the potential advancement of our early-stage programs. Any or all of the forward-looking statements may turn out to be

wrong or be affected by inaccurate assumptions we might make or by known or unknown risks and uncertainties. These forward-looking statements are subject to

risks and uncertainties including, without limitation, risks related to the success, timing and cost of our ongoing and anticipated clinical trials for our current product

candidates, including the timing of initiation, pace of enrollment and completion of trials, our ability to fully fund our disclosed clinical trials, which assumes no

material changes to our currently projected expenses, the receipt and timing of interim or preliminary results, which are not necessarily indicative of final results,

our understanding of product candidate mechanisms of action and interpretation of preclinical and early clinical results from our clinical development programs, our

ability to predict clinical outcomes based on such preclinical and early clinical results, the timing and outcome of regulatory interactions, and the other risks,

uncertainties, and factors described under “Risk Factors,” “Management’s Discussion and Analysis of Financial Condition and Results of Operations” and

elsewhere in the documents we file with the U.S. Securities and Exchange Commission. For all these reasons, actual results and developments could be

materially different from those expressed in or implied by our forward-looking statements. You are cautioned not to place undue reliance on these forward-looking

statements, which are made only as of the date of this presentation. We undertake no obligation to update such forward-looking statements for any reason, except

as required by law. This presentation shall not constitute an offer to sell or the solicitation of an offer to buy any securities, nor shall there be any sale of any

securities in any state or jurisdiction in which such offer, solicitation or sale would be unlawful prior to registration or qualification under the securities laws of any

such state or jurisdiction. This presentation may not be reproduced, forwarded to any person or published, in whole or in part.

APR-1051 and ATRN-119 are investigational drugs and have not yet been approved by the U.S. Food and Drug Administration or any other regulatory authority.

Aprea Therapeutics (NASDAQ: APRE)

One Critical Pathway - Multiple Targets

1. Ngoi N, et al. Targeting the replication stress response through synthetic lethal strategies in cancer medicine.

Trends in Cancer. (2021); 7(10):930-957

Precision

Medicine

Synthetic

Lethality Aprea

DYRK1

Target

Trends in Cancer 1

Positioned at the forefront of synthetic lethality and precision medicine

Transition from broad, toxic chemotherapy

to potentially safer, precision-guided

targeted therapies

Targeted Oncology

Develop highly selective cancer therapies

that exploit tumor-specific mutations to

maximize cancer cell killing while sparing

healthy tissue

Precision-Driven

Development

All programs are designed to address

significant unmet medical needs across

genetically defined cancer populations

Pipeline with

Clinical Momentum

Preliminary signals of monotherapy activity

observed in ongoing Phase 1 trial

Early Clinical

Proof-of-Concept

2025 2026 2027 2028

H2 H1 H2 H1 H2 H1 H2

Financing

WEE1

APR-1051

ATR

ATRN-119

(mosipasertib)

DYRK1

APR-1602

Robust DDR Development Pipeline and Corporate Milestones

2025-2028 Accomplished and anticipated milestones

DNA Damage Response (DDR)

*Planned major medical conference presentation

**FPI contingent upon execution of a research collaboration or partnering agreement; study initiation is expected to

be externally supported without use of current company resources.

RP2D for

QD dosing

ACESOT-1051: Phase 1 – Monotherapy Dose Escalation

Complete Dose

Escalation and Backfill

ACESOT-1051: Monotherapy Dose Selection Optimization

Safety &

Efficacy Data

Safety &

Efficacy Data

Phase 1/2a : Combinations to Expand Therapeutic Potential

Open Backfill

Cohorts

Lead Identification and Optimization

ABOYA-119: Phase 1/2a

Dose Escalation: QD/BID

Interim Safety &

Efficacy Presentation*

FPI**

IND Ready

$30 Million Raise

Enriched for USC and Cyclin E PROC

Combinations in HPV+ Head and Neck Cancer and Colorectal Cancer

Strong Drug Development and Commercial Expertise

Experienced team in synthetic lethality and targeted therapy

Management

Board of Directors

Richard Peters, MD, PhD

Chairman of the Board

Oren Gilad, PhD

President and CEO

Jean-Pierre Bizzari, MD

Director

Marc Duey

Director

Michael Grissinger

Director

Gabriela Gruia, MD

Director

John Henneman

Director

Rifat Pamukcu, MD

Director

Bernd R. Seizinger, MD, PhD

Director

Oren Gilad, PhD

President and CEO

John P. Hamill

SVP and CFO

Eugene Kennedy, MD

Chief Medical Advisor

Ze’ev Weiss, CPA,

BSc

Chief Business Advisor

Mike Carleton, PhD

Translational Medicine

Advisor

Brian Wiley

SVP, Corporate

Strategy

WEE1 Inhibitor: APR-1051

ACESOT-1051:

Clinical Proof-Of-Concept

WEE1 Has Emerged as a Therapeutic Target of Significant Industry Interest

1. Zentalis Corporate Presentation, April 2026

2. Debio 0123-101, A Phase 1 Trial of Debio 0123 In Combination With Carboplatin In Advanced Solid Tumors:

Safety, Pharmacokinetic, And Preliminary Antitumor Activity Data, Poster ASCO 2023

3. Abstract 3120, ASCO 2024

4. Poster CT022, AACR 2026

Clinically validated, prior WEE1 inhibitors have been challenged by narrow therapeutic windows

Aprea is applying key insights to advance APR-1051 as a potentially best-in-class WEE1 inhibitor

Program Clinical Limitations Strategic Outcome What It Signals

Adavosertib

(AstraZeneca)

Hematologic & GI toxicity limited

dose intensity

Terminated further clinical development

Returned by AstraZeneca to

Merck & Co.

Biology works, narrow

therapeutic window

Azenosertib

(Zentalis) Continuous dosing not tolerated1 TRAEs leading to dose reductions,

interruptions and discontinuation

Biology works, therapeutic

window still being defined

Debio 0123

(Debiopharm)

QT prolongation liability at high

doses2

Limited single-agent activity – no

responses up to MTD3; activity seen in

combination with PKMYT1 inhibitor4

Potential future as

combination agent

No head-to-head studies have been conducted. Trial information is based on publicly available data and should be interpreted cautiously

Program Engineered Profile Strategic Outcome What It Signals

APR-1051

(Aprea Therapeutics)

Structurally differentiated, highly

potent, limited off-target

inhibition

Preliminary signals of monotherapy

activity without class-limiting tox to date

Potential to expand

therapeutic window

TRAEs – Treatment related adverse events

ACESOT-1051: Phase 1 Study Design

Multi-center, open-label Phase 1 single-agent dose escalation and dose selection optimization

Part 1 Dose escalation

up to 100 patients

RP2D

Oral single-agent APR-1051 will be administered once-daily for 28-day cycles

Objectives

Primary: Safety, DLT, MTD/MAD, RP2D

Secondary: Pharmacokinetics, Antitumor activity (RECIST/PCWG3)

Exploratory: Pharmacodynamics

= cleared

Accelerated titration; 1-6 patients per dose level

BOIN design; 3-12 patients per dose level

Part 2 Dose selection optimization

Up to 80 patients

Selected

dose 2

Selected

dose 1

1:1

randomization

Dose level 1-3

10 – 30 mg

Dose level 4

50 mg

Dose level 5

70 mg

Dose level 6

100 mg

Dose level 7

150 mg

Dose level 8

220 mg

Dose level 9

300mg

Confirmed

PR Observed

First uPR

Observed

Select 2

dose levels

Eligible patients

≥ 18 yo with advanced solid

tumor harboring cancer-associated gene alterations

o USC regardless

biomarker status

o Cyclin E-overexpressing

platinum-resistant

ovarian cancer (PROC)

o CCNE1, CCNE2, FBXW7

or PPP2R1A

o HPV+ oropharyngeal

squamous cell carcinoma,

cervical, vaginal, or vulvar

carcinoma

o KRAS-GLY12/GLY13 &

TP53 colorectal cancer

= currently enrolling

Backfill

Dose level 10

400mg

Dose level 11

500mg

Backfill

223

178

112

181

140

0 50 100 150 200 250

01-005

03-005

02-003

03-006

01-006

03-007

03-008

01-008

01-010

03-010

03-013

01-014

03-011

01-011

03-012

01-012

01-013

01-015

02-004

300 mg

220 mg

150 mg

100 mg

70 mg

Days on treatment

Study patient

✼

APR-1051 once-daily dose

Not all data source verified

Data Cutoff: May 6, 2026

APR-1051 Summary of Duration of Treatment (n=28 for all dose levels)

✼ Progressive disease (PD)

Stable disease (SD)

Consent withdrawn

Treatment continues

Unrelated death

Partial response (PR)

Enrollment status

✼

Not shown:

50 mg (n=6)

30 mg (n=1)

20 mg (n=1)

10 mg (n=1)

MedDRA Preferred Term APR-1051 All dose levels (N=28)

Treatment-related AEs, n (%)a All Grades Grade ≥ 3b

Nausea 10 (35.7) 0 (0)

Fatigue 4 (14.3) 0 (0)

Vomiting 3 (10.7) 0 (0)

Alanine aminotransferase increased 1 (3.6) 1 (3.6)c

Anemia 1 (3.6) 0 (0)

Aspartate aminotransferase increase 1 (3.6) 1 (3.6)c

Blood bilirubin increased 1 (3.6) 0 (0)

Constipation 1 (3.6) 0 (0)

Dehydration 1 (3.6) 0 (0)

Dysgeusia 1 (3.6) 0 (0)

Dyspepsia 1 (3.6) 0 (0)

Eczema 1 (3.6) 0 (0)

Gastroesophageal reflux disease 1 (3.6) 0 (0)

Hypokalemia 1 (3.6) 0 (0)

Lymphocyte count decreased 1 (3.6) 1 (3.6)

Platelet count decreased 1 (3.6) 0 (0)

Treatment-related AEs in Patients Treated with APR-1051 (N=28)

a A patient may have more than one AE and/or have the same AE more than once

b Grade 3 unless otherwise indicated

c Increased alanine aminotransferase and aspartate aminotransferase occurred in the same patient, was determined to be serious, and considered one DLT event

Not all data source verified

Data Cutoff: May 6, 2026

Confirmed Partial Response in Patient 01-011

220mg QD – Currently on treatment

Demographics: 63-year-old Black Female

Site: MD Anderson Cancer Center

Diagnosis: Uterine carcinosarcoma

Key Mutations: PPP2R1A

Treatment History (4 prior lines)

• Line 1: Carboplatin + Paclitaxel → 126 days, PD

• Line 2: Doxorubicin → 56 days, PD

• Line 3: Topotecan → 70 days, PD

• Line 4: Pembrolizumab + Lenvatinib → 5months, PD

APR-1051 – Treatment Outcome

• C1D1: Dec 18, 2025

• Current Status: On treatment 140 days (C6D1) – May 6, 2026

• Best Response: Confirmed Partial Response (PR)

• PR (-50%) at first assessment Feb 10, 2026

• Confirmed PR with additional -9.5% reduction from C3D1 Mar 10, 2026; scan April 8, 2026 showed 0% change

• Tumor marker: CA-125 reduction from BL 362.4 U/mL to C3D1 46.8 U/mL (87% decrease); 40.2 U/mL Mar 11, 2026; 53 U/ml April 08, 2026

• Adverse Events: C1D22 Gr 1 rash. C1D15 Gr1 thrombocytopenia , possibly related to IP; intermittent nausea Gr1 probably related; amylase

increase Gr1 unlikely related. No DLT

Disease Control Observed in Early Patient Outcomes

APR-1051 shows preliminary evidence of disease control in rectal cancer with mutated FBXW7

100 mg Cohort Case Report

Patient: 86-year-old Asian Female

Diagnosis: Rectal Cancer

Key Mutations: FBXW7 (Drives Cyclin E accumulation and overexpression)

Treatment History: 5 prior lines - heavily pretreated

• Line 1: Capecitabine/oxaliplatin → 191 days, PD

• Line 2: Capecitabine/oxaliplatin/bevacizumab → 45 days, PD

• Line 3: FOLFIRI + bevacizumab → 43 days, PD

• Line 4: Local XRT (lung mets) → 12 days, not evaluable

• Line 5: Tretinoin/bevacizumab/Tecentriq (ATRT trial) → 50 days, PD

APR-1051 Activity:

• Current Status: Consent withdrawn after 178 days

• Best Response: SD at third scan (-15% tumor response)

Notes: Durable SD maintained 181 days in a heavily pretreated 86-year-old patient; well tolerated with minimal toxicity.

FBXW7 mutation may be relevant to response

Stable disease maintained for 178 days in patient with FBXW7 mutation (100 mg QD) (elected to stop study participation)

70 mg Cohort Case Report

Disease Control Observed in Early Patient Outcomes

Patient: 62-year-old White Male

Diagnosis: HPV+ Oropharyngeal Squamous Cell Carcinoma (base of tongue)

Key Mutations: P16+

Treatment History: 3 prior lines

• Line 1: Concomitant cisplatin/XRT → 49 days, PD

• Line 2: Pembrolizumab→ 84 days, PD

• Line 3: Paclitaxel/carboplatin → 184 days, PD

APR-1051 Activity

• Current Status: PD after 223 days of SD treatment

• Best Response: SD at first scan (-5% tumor response)

Notes: Stable disease maintained for 223 days.

APR-1051 shows preliminary evidence of disease control in HPV+ head and neck cancer

Stable disease maintained for 223 days in patient 01-005 HPV+ head and neck cancer (70 mg QD) (PD)

Biomarker Defined Registration Path

*Data as of May 6, 2026 and are preliminary from an ongoing dose-escalation study. Responses and stable disease

require confirmation in additional patients and may change as follow-up matures. Safety and efficacy outcomes may

vary by dose, schedule, and patient characteristics.

Preliminary signals of monotherapy activity across cohorts in genomically defined tumors including

uterine cancer, endometrial cancer, CRC and HNSCC

• Responses across dose levels support a biomarker enriched expansion path

• Activity beyond endometrial cancer supports additional biomarker-defined cohorts

Clinical Activity by Indication and Biomarker Path to Registrational Cohort

1 Expand USC and CycE-PROC

Add additional cohorts

• CRC FBXW7-mutated

• HPV+ cancers

• PPP2R1A mutated

Confirm durability, consistency of

response and safety 3

Indication Biomarker / Altered

Genes

Clinical Activity in

ACESOT-1051*

Uterine/

Endometrial

Cancer

PPP2R1A

2 PR

CCNE1 overexpressed 2 SD

TP53

CRC

KRAS & TP53

3 SD

FBXW7

HNSCC HPV+ 1 SD

APR-1051:

Potentially Differentiated WEE1 Inhibitor

Pre-Clinical

APR-1051: Potentially Best-in-Class WEE1 Inhibitor

Structurally differentiated: high potency, limited off-target inhibition design compared to other molecules

AstraZeneca

Adavosertib (AZD-1775)

Zentalis

Azenosertib (ZN-c3)

Aprea

APR-1051

Undisclosed

APR-1051 is based on a different molecular structure than AZD-1775 and ZN-c3 (not an analogue)

No head-to-head clinical studies have been conducted.

APR-1051: Potentially Best-in-Class WEE1 Inhibitor

Potent inhibitor of WEE1

Does not substantially inhibit structurally and functionally related PLK1, PLK2 or PLK3

-10 -9 -8 -7 -6 -5 -4

100

125

PLK1 IC50 Determination

Log10 [conc] (M)

% Activity

ZN-c3

APR-1051

-10 -9 -8 -7 -6 -5 -4

100

125

PLK2 IC50 Determination

Log10 [conc] (M)

% Activity

ZN-c3

APR-1051

-10 -9 -8 -7 -6 -5 -4

100

125

PLK3 IC50 Determination

Log10 [conc] (M)

% Activity

ZN-c3

APR-1051

ZN-c3 = 92.1 nM

APR-1051 = 15,900 nM

PLK1 Inhibition

IC50 >150-fold difference

ZN-c3 = 32.0 nM

APR-1051 = 1,800 nM

PLK2 Inhibition

IC50 >50-fold difference

ZN-c3 = 52.2 nM

APR-1051 = 31,600 nM

PLK3 Inhibition

IC50 >600-fold difference

Important difference in off-target inhibition between APR-1051 and ZN-c3 On 1

-target WEE1 potency1

ZN-c3 = 2.9 nM

APR-1051 = 1.6 nM

WEE1 Inhibition

IC50 similar to ZN-c3

125

100

10 9 8 7 6 5

APR 1051

WEE1 IC50 Determination

% Activity

Log10 [conc] (M)

AACR-NCI-EORTC Meeting, Poster B323, 2024

1Data from exploratory in-vitro studies. No head-to-head clinical studies have been conducted.

APR-1051 specificity for WEE1 opens potential for greater therapeutic window

PLK1 Inhibition Counteracts Effect of WEE1 Inhibitors1

Minimal PLK1 co-inhibition enhances therapeutic window for APR-1051 37 nM 111 nM 333 nM 1 µM 37 nM 111 nM 333 nM 1 µM

Phos-H2AX

Control

(MCM3)

Phos-CDK1

APR-1051

75 nM

GSK-PLKi

Control

(MCM3)

37 nM

111 nM

333 nM

1 µM

37 nM

111 nM

333 nM

1 µM

Phos-H2AX

400 nM

BI-PLKi

APR-1051

Phos-CDK1

PLK inhibitor, BI-2536, interferes with the

effects of APR-1051 in OVCAR-3 cells

1 AACR-NCI-EORTC Meeting, Poster B323, 2024 Data from exploratory in-vitro studies

Evidence of DNA damage

allowed by WEE1 inhibition.

PLK1 reduces functional

potency of WEE1 inhibition

PLK inhibitor, GSK-461364, interferes with

the effects of APR-1051 in OVCAR-3 cells

Inhibition of PLK1 reduces efficacy of WEE1 inhibition. Results in requiring higher

doses of WEE1 inhibitors and introduces PLK1 related toxicity

Studies Show PLK1 Suppression is Associated with Sepsis-Induced

Loss of Intestinal Barrier Function

1 PLK1 protects against sepsis-induced intestinal barrier dysfunction, Cao et al, Scientific Reports (2018).

2 PLK1 protects intestinal barrier function in sepsis: A translational research, Cao et al, Cytokine (2023).

3 PLK1 protects intestinal barrier function in sepsis: A translational research, Cao et al, Molecular Medicine (2022).

4 LncRNA DANCR improves the dysfunction of the intestinal barrier and alleviates epithelial injury by targeting the

miR‐1306‐5p/PLK1 axis in sepsis, Wang et al., Cell Biology International (2021).

HPV+ Cancer – Collaboration with MD Anderson Ovarian Cancer*

UMSCC47 tumor cells OVCAR3 tumor xenograft

Tumor Volume (mm3

)

Vehicle

Aprea ATRi 30 mg/kg QD

Aprea WEE1i 30 mg/kg QD

Combination – half dose,

15mg/kg each

Days Post Treatment

Tumor Volume (mm3

)

Days since start of xenograft

mEER tumor cells

Tumor Volume (mm3

)

Days since start of xenograft

APR-1051 Showed Preclinical Activity in Combination with Chemo, IO

and ATRi Across Multiple Cancer Models

* Data on file.

Start of

treatment

rea ed

Cis a i

Start of

treatment

** P<0.0001

* P=0.0067

** *

Chemotherapy Immuno-oncology DDR combination

APR-1051 suggests synergistic potential preclinically with standard oncology agents

APR-1051 WEE1 Summary

A next-generation WEE1i with the potential to deliver meaningful clinical benefit and durable value

Clinically validated target

• WEE1 inhibitors have shown promising activity in genomically defined tumors

• Competitor programs constrained by low therapeutic window

APR-1051 clinical highlights

• Preliminary clinical proof-of-concept at 150 mg and 220 mg dose levels with two partial

responses and six patients with stable disease presented at ASCO 2026

• Potentially favorable safety profile at active dose levels

• Enrollment accelerating with expansion from three to ten active clinical sites

• Interim clinical data to be presented Q4 2026

APR-1051 preclinical differentiation and potentially best-in-class opportunity

• Novel structure with high potency, limited off-target inhibition design

• Minimal PLK1 inhibition enhances therapeutic window

• Potential for synergy demonstrated in combination with standard oncology agents

Intellectual Property Portfolio

Financial Summary & Capitalization

Investment Highlights

Robust Global Intellectual Property Protection

Family 1 Ataxia Telangiectasia and

Rad3-Related (ATR) Protein

Kinase Inhibitors

• Macrocyclic inhibitors of ATR & methods of using them to treat various cancers, filed on Oct. 13th, 2015

• Patents granted in AU, BR, CA, CN, EP, IL, IN, JP, KR, MX, HK.

• 1.1: Issued on May 30, 2017 as U.S. Patent 9,663,535; 1.2: Issued on May 29, 2018 as U.S. Patent 9,981,989; 1.3: Issued

on Feb. 5, 2019 as U.S. Patent 10,196,405

Family 2 ATR Inhibitors and Methods

of Use

• Carboxylic acid-containing macrocyclic ATR inhibitors, and prodrugs; methods of using these inhibitors to treat various

cancers; filed on Apr. 12, 2017

• Issued on May 28th, 2019 as U.S. Patent 10,301,324

Family 3 ATR Inhibitor Pharmaceutical

Composition and Methods

• International application filed on Apr. 14, 2023

• Pharmaceutical formulation and composition of our lead ATR inhibitor in the clinic

• Patent granted in JP and EA; Applications pending US, AU, BR, CA, CN, EP, HK, IL, IN, KR, MX, NZ, PH, SG, ZA

Family 4 WEE1 Inhibitor

Pharmaceutical

Compositions and Methods

• International Application filed on Jun. 3, 2022

• Composition of our lead WEE1 inhibitor compounds

• Patent granted in AU; Applications pending in US, AU, BR, CA, CN, EP, HK, IL, IN, JP, KR, MX, ZA

Family 5 Methods of Treating Cancer • Clinical methods of treating advanced solid cancer tumors using lead ATR inhibitor

• International application filed on Sept. 19, 2025

Family 6 Macrocyclic DYRK1A-B

Inhibitors and Methods of

their Preparation and Use

• International application filed on Jan. 22, 2026

• Macrocyclic inhibitors of DYRK1A-B and methods of using them to treat disease

Family 7 Macrocyclic DDR Target

Inhibitors and Methods of

their Preparation and Use

• International application filed on Jan. 25, 2026

Family 8 Methods of Treating Cancer • Provisional application filed on Jan. 9, 2026

• Clinical methods of treating cancer using lead Wee1 inhibitor

Securities Common Equivalents

as of August 12, 2026

Preferred Stock (as converted) 15,596

Common Stock (1) 12,591,136

Warrants (2) 89,438,517

Options 1,137,333

Restricted Stock Units 65,438

Fully Diluted Equivalents 103,248,020

Aprea Therapeutics (NASDAQ: APRE)

1. 400,000,000 common shares authorized

2. Total warrants include pre-funded, Tranche A, Tranche B and Purchase

Cash and Equivalents of ~$41.2M as of June 30, 2026

$30.0M in gross proceeds raised in private placement that closed on March 31, 2026

Financial Summary and Capitalization

Expected cash runway into Q1 2028

• Achieve near term inflection points and catalysts

• Evaluate optimal strategic partnerships

Near term catalysts

• APR-1051: Q4 2026 Interim clinical data; Q2 2027 Complete dose escalation

• ATRN-119: October 2025 RP2D ✓ H2 2026 Potential collaborations on combinations

Highly potent and selective design, potential best in class inhibitors

• Diversified portfolio including WEE1 (APR-1051) and ATR (ATRN-119) inhibitors

• Preliminary evidence of clinical activity including PRs with APR-1051

• Single agent and combination potential therapies

Technology developed by pioneers in synthetic lethality

• Management with strong drug development and commercial expertise

• Focused on addressing unmet needs for patients with biomarker defined cancers

Investment Highlights

Aprea Therapeutics

(NASDAQ: APRE)

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- Definition

Boolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Exchange Act

-Number 240

-Section 13e

-Subsection 4c

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- Definition

Boolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Exchange Act

-Number 240

-Section 14d

-Subsection 2b

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- Definition

Title of a 12(b) registered security.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Exchange Act

-Number 240

-Section 12

-Subsection b

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- Definition

Name of the Exchange on which a security is registered.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Exchange Act

-Number 240

-Section 12

-Subsection d1-1

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- Definition

Boolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as soliciting material pursuant to Rule 14a-12 under the Exchange Act.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Exchange Act

-Number 240

-Section 14a

-Subsection 12

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- Definition

Trading symbol of an instrument as listed on an exchange.

+ References

No definition available.

+ Details

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- Definition

Boolean flag that is true when the Form 8-K filing is intended to satisfy the filing obligation of the registrant as written communications pursuant to Rule 425 under the Securities Act.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef

-Publisher SEC

-Name Securities Act

-Number 230

-Section 425

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